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Dna Quantification Of Blood Stains Recovered From Washed Fabrics

by Zeenat Salah-ud-din | Dr. Muhammad Yasir Zahoor | Dr. Muhammad | Dr. Muhammad Wasim.

Material type: book Book; Format: print ; Literary form: drama Publisher: 2013Dissertation note: Bloodstains are extremely important forensic evidences for DNA profiling. Bloodstains recovered at crime scene can help to identify and confirm the credentials of the victim. Criminal can make all efforts to destroy and hide possible evidence at crime scene therefore these bloodstains on the crime place can hardly provide any evidence against the culprit. The criminal will try to take away or change clothes with the bloodstains of the victim. Once perpetrator has removed the bloodstains, culprit will feel secure and satisfied. But as a suspect if clothes are recovered with washed bloodstains and forensic evidence of DNA of the victim can be recovered from the washed clothes and can provide strong evidence against suspect. Present study has been planned to quantiate DNA from 10 different types of bloodstained fabrics with possible ways of washing to hide the blood from fabrics. 100 ìl of preservative blood was used in creating blood spot on fabric. After that dried bloodstained fabrics were hand washed with tap water and in detergent for 5 and 10mins and air dried at room temperature (25°C- 30°C) in laboratory. DNA was extracted through Chelex® 100 extraction procedure from these fabrics and analyzed on Real time PCR kit i.e. Quantifiler™ Human DNA Quantification kit which is specific for Human DNA. Research study showed there was no statistically significant difference in DNA concentrations among different washing methods (p-value = 0.318). There was statistically significant difference in DNA concentrations among different types of fabrics divided into two categories (p-value = 0.001, 0.002). Research findings showed that cellulose fibers recovered highest quantity of DNA, followed by artificial fibers and then protein fibers (cellulose >artificial>protein fiber) despite their washing methods. Thus provides a lead in solving such type of criminal cases. Availability: Items available for loan: UVAS Library [Call number: 1663,T] (1).

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Deoxyribo Nucleic Acid Extraction & Qantification From Human Saliva Deposited On Fruits With Human Bite

by Shahid Nazir | Dr. Muhammad Wasim | Dr. Muhammad Yasir Zahoor | Ms. Faiza.

Material type: book Book; Format: print ; Literary form: drama Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1717,T] (1).

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Allele Frequency Distribution Of 15 Star Loci In Gujjar Cast Of Punjab Pakistan

by Maira shakoor | Dr. Muhammad Yasir zahoor | Dr. Tanveer hussain | Ms. Shagufta.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2077,T] (1).

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